I have the API test kit that includes two bottles of solution. It says to add 8 drops of each to 5ml of water and allow 5 minutes before checking. I do this and according to the test the tank I'm cycling has 0 ammonia, but I left the water in the test tube and after 25 min or so it was showing a small amount of amonia.. light green instead of yellow. So would you think it's no ammonia or a small amount of ammonia? I would think none but then why would it change after an additional 20 min? And yes.. I would be a sucky chemist