Just took all the fry from my 20 tall and put them in a breeder net in my 75 until I can get them to my LFS within a few days. I'm going to out my Greenside darters in the 20 tall which I'm making a riffle tank. I'd prefer to wait until scaped the tank before putting the darters in to avoid stress but I still need to get rocks and substrate from my local creek and wash it all before I can start to scape. Which is fine with me but I don't know how long the tank will stay cycled without an ammonia source in it and I don't want to have to recycle it. So how long will the bacteria live with no fish? There is still some waste floating around but I don't know of that'll help keep it cycled.