Zoodiver;3546110; said:
There are rumors floating around about barb removal. None of them that I know of are true. I have yet to see a ray live long term after having the barb cut out of it's tail in a surgical procedure.
I got a
Potamotrygon leopoldi in that, sadly, had been the victim of a severe bite to his tail. When I opened up the bag, his tail was still a little ragged.

With some clean water, a steady diet and some nice R&R he healed over in a matter of days. He is completely without a barb, and it is a really sad thing to see. He is such a beautiful animal, and then I see the end of his tail and it just kills me.
After he healed up and got to a healthy size, I introduced him into one of my larger tanks. Unfortunately he was assaulted by my largest male (because of what I am guessing was misplaced sexual interest) and his tail wound was bitten again. This most recent time his tail has healed, he regrew a very small and awkwardly angled barb. It fell out, and in the five or so months since this last incident he has healed up wonderfully, entirely without a barb. He is now happily swimming around in our smaller 360 gallon tank, waiting to grow big enough to attempt to re-introduce to my larger male population.
I strongly recommend never intentionally putting your animals through this. Even if you believe that in principal this could be acceptable to do (similar to de-clawing cats), we just do not know enough about these animals and their physiology to ensure that your specimen will recover fully, much less survive the process. It just seems so unreasonable to take such a senseless risk with such a beautiful, and often expensive, animal.