I had just completed constructing my 1230 gals concrete pond. I installed it with a 55-gal plastic barrel biofilters and a RIO 12HF Submersible Pump (rated at 630 gals per hour with a max head of 10 ft or 3m). It is common knowledge that before you introduce your koi to the ponds, the bacteria colony (inside the biofilter) must be established first in order to process the ammonia and nitrites discharge of the koi. I have no idea as to how many days or months and the procedures to colonized these bacteria that will process the ammonia and nitrite thus through this forum, I asked recommendation/suggestion.