It does. Thanks.
So, under normal situations (a cycled tank), most of your BB would be in your sump. The first chamber would be loaded as the sponge, jap mat and matrix would be well inhabited, and the mature K1 would have plenty.
I'm concerned that the seeded amount died off however. (I assume you were referring to the K1.) It makes wonder if most of the tank's BB was wiped out at some point. Have you been testing recently and if so are you getting 0 ammonia, 0 nitrite and 5-30 for nitrate?
Is it possible (since this is at work out of your control) that something happened out of your sight? Your tank is not over stocked, and you don't appear to be over feeding. No reason to have spikes unless some one else is feeding a lot (in your absence) or the BB was killed off.