What skjl47 says is very very true. I think everyone who has an aquarium, no matter what size, will have a dedicated area for bio media whether it's in a section of a sump or a cannister filter or whatever. This seems to be the law of aquaria, you must have a bio media area. No you don't. I've been tinkering with one of the sumps on my 180's for the past 18 months or so. Initially my central chamber was stuffed with bags of ceramic rings, plastic bio balls and old off cuts of coarse sponges.
I soon realised that just because i have a dedicated area for my BB to grow, does that mean that this is the only area that they live? Absolutely not. BB are all over your tank. I'm betting that there's as much on the surface of your glass/acrylic, substrate, decor and inside drain and return line pipework than what there is in any dedicated bio media area.
This is something i've been proving to myself for the past 18 months. Everytime i do a water change i'll take a bit of bio media out of the mid section of my sump. Presently my mid section is very sparse on one of my 180's now, my other 180 i haven't touched yet, and i wont until i prove to myself that i can run my experimental tank bio media free. Thus far i haven't had a single issue with parameters, no crashes, mini cycles or anything. Maybe i've just been extremely lucky, i don't think so.
Meanwhile my experiment will continue.