-has been done in aquaria even with just two individuals; rarely occurs though
-fish cannot be externally sexed by conventional methods. info on age and species can help make an educated guess, however. there are recent advancements in blood analysis which would allow sexing the fish without killing them.
-spotted gars can mature after their first year, often males at 2-3, females at 3-4. longnoses take a bit longer. tropicals mature earlier than cubans and gators, with gator females often not maturing until age 11.
hope those details help a bit--
--solomon