Usually you have to have a creature in their to start the ammonia cycle..........You have a couple in there, right??????????????Tell you the truth, I have been running my tanks on a system startup for about 20 years now with no flaw.............I cycle the water for about 10 days with a couple feeder fish, medium sized goldfish or COnvict Cichlids......I also have snails running around the system, inside the tank and inside the filter..........After about a week and a half to two weeks, I get my new fish.....................