i'm read you should add anywhere from 4ppm (parts per a million/ mg per a litre) to 6ppm pf ammonia. I aimed for 5ppm in my 225 us gallon tank. In the middle of the cycle right now, ammonia is done, waiting for nitrites to spike and zero off. I was using a 9% solution of ammonia and it took about 13-14 tea spoons to reach the right concentration for me.