Ive always kind of wondered this when doing water changes or filter maintainence...for example on my 125 I have 3 filters (2 xp4s and 405)..I obviously never clean them at the same time...when I do clean one I take out all the baskets and then put the biomedia in a small bucket and run tank water over it...the baskets are then put back in (dry) and stay that way for a few minutes or more until I can reconnect the filter...my long drawn out question is (lol)...when does the beneficial bacteria die or does it when dried out for awhile? I have so much filtration on the tank that I have to clean my filters often or my nitrates are skyhigh...but im worried all the cleaning doesnt give the BB enough time to "re-seed" in the filter...any thoughts?