So what your overall conclusion?
Out of 50 TSN:
-- 48 fasciatum or fasciatum hybrids and
-- only 1 (supposed) reticulatum and
-- only 1 (supposed) tigrinum?
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I didn't know you went through that many, thought ~25-30. Ok, I stand corrected. One needs to collect 1000 TSN, not 100. And exclude all the deformed ones!
Now that's a project not for the faint of heart.