Astronotus study-- effect of prebiotics

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neutrino

Goliath Tigerfish
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Jan 22, 2013
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(note-- pre--biotics, not probiotics)
https://www.researchgate.net/public...dietary_administration_of_xylooligosaccharide

Prebiotics are naturally occurring in certain foods-- in some seaweeds and algae, besides the sources mentioned below. This leads me to think some commercial fish foods already have them, some products more than others by virtue of the ingredients in them. To my thinking this illuminates advantages of some products over others and is an argument against exclusively feeding certain ingredients, as well as some home recipes for fish foods, at least if you're interested in fish intestinal health. Theres more to good nutrition than adding some vitamin mix to a batch of beef heart.

https://www.hindawi.com/journals/grp/2016/5789232/
Prebiotics can nourish probiotics and encourage them to function more efficiently, allowing the bacteria to stay within a healthy balance [3]. They are nondigestible food ingredients, typically oligosaccharides that serve as the fuel for probiotics, allowing these beneficial microorganisms to thrive by going through the fermentation process [3]. Some of the commonly known prebiotics are fructooligosaccharides, galactooligosaccharides, and lactulose [6–8]. In addition, other types of oligosaccharides, such as isomaltooligosaccharides (IMO) and XOS, are emerging as a potential novel source of prebiotics that can be used as functional ingredients in foods [9, 10]. Of the emerging prebiotic oligosaccharides, XOS have attracted increasing interest because of their health, physicochemical, and technological related properties. XOS are mixtures of oligosaccharides containing β-1,4-linked xylose residues which naturally occur in bamboo shoots, fruits, vegetables, milk, and honey...
 
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(note-- pre--biotics, not probiotics)
https://www.researchgate.net/public...dietary_administration_of_xylooligosaccharide

Prebiotics are naturally occurring in certain foods-- in some seaweeds and algae, besides the sources mentioned below. This leads me to think some commercial fish foods already have them, some products more than others by virtue of the ingredients in them. To my thinking this illuminates advantages of some products over others and is an argument against exclusively feeding certain ingredients, as well as some home recipes for fish foods, at least if you're interested in fish intestinal health. Theres more to good nutrition than adding some vitamin mix to a batch of beef heart.

https://www.hindawi.com/journals/grp/2016/5789232/

Thx for sharing Neutrino, interesting stuff. In fact about a month ago I was delving into this subject a bit when I was researching about the ganoderma polysaccharides (from a fungus/mushroom that goes back ages in Chinese medicine). I learned that these are prebiotics, that besides the growth benefits you mention above, have also been proven in quite a few studies to be very effective anti-oxidants and immunostimulants (in both fish and humans).

The food I was referring to was Azoo's premium Ultra Fresh line:
1380339

We were talking about this in a thread (https://www.monsterfishkeepers.com/...glish-translation-of-food-ingredients.716684/) in the Arowana sub-forum, here's one post with some interesting links:

There are actually quite a few other sources of 'fungal polysaccharides' besides lingzhi. From another interesting study done in 2018:
Potential uses of fungal polysaccharides as immunostimulants in fish and shrimp
aquaculture: A review (https://www.researchgate.net/public...lants_in_fish_and_shrimp_aquaculture_A_review):


”Higher fungi are well sources of a wild range of most important natural products.
Over the last decade, great attention was generated in the polysaccharides produced by the
numerous fungi especially mushrooms because of their various biological properties, such as
anti-oxidant, anti-cancer, anti-microbial, immunostimulation and hypoglycemic activities
(Cohen et al., 2002) (Fig. 1). Many higher Basidiomycetes mushrooms (Grifola frondosa,
Lentinula edodes, Schizophyllum commune, Coriolus versicolor, Pleurotus ostreatus and
Ganoderma lucidum) and Saccharomyces cerevisiae contain bioactive polysaccharides in its
fruiting bodies, mycelium and broth culture (Wasser, 2002).


To be clear I'm not trying to come across as Mr. Scientist in the know -- I'm learning more about this stuff too -- but here's some more interesting info about these fungal polysaccharides, which I've learned are used as a prebiotic (which is essentially food for probiotic bacteria in simple terms).

"Polysaccharides are prebiotic substances which are boosting the immune responses,
resistance to pathogens and growth performance of fish and crustaceans (Bai et al., 2015;
Ringo et al., 2010; Mohan et al.,2016). Prebiotics are defined as indigestible dietary components that are utilized by specific health-promoting bacteria. These bacteria are considered beneficial to the health and growth of the host by reducing the occurrence of intestinal pathogenic microbes (Roberfroid, 1993; Gibson and Roberfroid, 1995; Gibson, 1998; Manning and Gibson, 2004). Fungal polysaccharides activate the innate immune system in two ways, by direct method and by improving the growth of intestinal microbiota.”


And their conclusion:
Highlights
-- The potential uses of fungal polysaccharides (prebiotics) on fish and shrimp aquaculture was investigated.
-- Dietary supplementation of fungal polysaccharides can enhance growth and innate immune responses of fish and shrimp.
-- Dietary supplementation of fungal polysaccharides can enhance the ability of fish and shrimp to resist the microbial infection.


Another interesting study -- The Role of Prebiotics and Probiotics in Aquaculture (http://www.global-engage.com/life-science/prebiotics-probiotics-synbiotics-aquaculture/) also points out that prebiotics like fungal polysaccharides can be used to offset some potential down-sides of large scale use of probiotics -- which RD. RD. touches upon in his well-known thread about probiotics: https://www.monsterfishkeepers.com/...robiotics-in-aquaculture.536531/#post-6415196

This approach of balancing prebiotics and probiotics is called "synbiotics", and it could be one of the best ways going forward to get away from use of antibiotics and harmful chemicals:
"Work using Pangasianodon hypopthalmus as a model and live yeast as probiotic, combined with selected prebiotics, showed improved growth performance, digestibility of diets containing high soybean protein and fish health, compared to yeast used alone."

One big problem though as the study points out is the higher cost of prebiotics -- so until this changes one shouldn't expect to find GLP in any cheaper, lower quality fish foods.

For sure I'm not as knowledgeable about this bio-science stuff as guys like you and RD, but nonetheless found the info very interesting.
 
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I found the researchers 70% per day water change schedule more interesting than the diet results.
"static aerated water..." the summary says, which got my attention the first time I read it. Apparently, rather than cycling (or buying) a filtration system for multiple tanks in the test, they did the daily water changes-- you'd have to with "static aerated water". In any case, the only difference between the groups was nutrition, making that the significant variable between groups in the study.
 
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For sure I'm not as knowledgeable about this bio-science stuff as guys like you and RD, but nonetheless found the info very interesting.
Not that I'm an expert on it, I just follow my nose when I find something interesting, especially if it's something relevant to my fishkeeping-- or my other science interests. :)

Interesting link in your post above. Section on salmonids and bactericidal and tumor effects was interesting. Some years ago I had read a little about a fungus derived anti-bacterial and fish-- would have to think about it to try and get the name to come to me, some fishkeepers had apparently experimented with it.

Interesting that shrimp have only an innate immune system. Didn't know that, never thought about it. Fish (and mammals) have both-- innate and adaptive immune systems-- which is where I don't agree totally with the theory that fish can deal with pathogens found in their home waters because they've had 'millions of years to adapt to them' but can't deal with new pathogens. Doesn't quite work that way. The whole point of an adaptive immune system is it's a means of dealing with novel pathogens-- it's the fundamental reason vaccination works, exposure to a less virulent version of a pathogen 'teaches' the immune system to deal with it.

When you do some reading on what adaptive immune systems do, it's really brilliant-- a learning system, essentially... reminds me of 'artificial intelligence' software, except biochemical. Not that it doesn't have its limits in fish, mammals, or humans-- cancers, for example.
 
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I have a subscription to the following digital magazine, All About Feed, which includes a number of regular contributions regarding aquaculture feed ingredients. Post the antibiotic era, prebiotics, probiotics, and various other feed additives are commonly discussed.


I find it all very interesting.
 
One part of the positive effect of prebiotics on intestinal health is the agglutination of pathogens. Harmful bacteria like Vibrio spp. are bound in complexes and discharged.
Sounds like Acurel for the gut. :-)
 
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