Congrats on the hold! It's not uncommon for females to loose their first few holds. It takes them a couple tries to get it right. Just make sure the male isn't harassing her too much.
As far as the twitching, several of my mbuna females will do it to each other to show who's boss. The males will do it mostly for breeding but sometimes will show off to a new fish to show they are in charge.
As far as the twitching, several of my mbuna females will do it to each other to show who's boss. The males will do it mostly for breeding but sometimes will show off to a new fish to show they are in charge.