Hello; My take is that the bb are on surfaces thru out a tank with likely higher concentrations of colonies where there is water flow. I think this because the bb "feed" on ammonia and water flow should be more likely to carry the ammonia across some surfaces more than others. The power filter parts will be one of these areas. I consider the tubes, boxes and such of a filter to be as likely to have bb colonies as the filter media.
The substrate should have bb colonies but might be more restricted to areas near the top and not so much deeper in the substrate where water flow may be much less. Exceptions may be those of us who still use the UGF's ( undergravel filters) where a water flow is created into the substrate. large fish that plow up the substrate may encourage more growth of bb colonies.
Fine substrates like sand are reported by members to be prone to growth of anaerobic bacteria if the depth is over an inch. This seems to me to indicate that the bb likely are not be found deep in such substrates.
My take is the bb are on many surfaces in a tank as well as the filter media and substrate.
Thanks for the info.
Not to jack this thread, but this raises a question on my tanks. I use a combo of sand, crushed coral, and small pebbles. It is over an inch in depth. Is that too much?
I have earth eating cichlids mostly, and they are constantly picking a the surface of the substrait, but never go too deep. Wouldn't deep sand trap more waste that isn't being eaten by the bacteria? I have always thought this so when I do water changes, I stir up the sand after I get the waste on the surface of the sand.