man, john was right, they dont cross. ive had guppy ponds for years now, the guppies and gambusia never ever cross bred.
and they are already cold tolerant. they handle our winters here down to a few degress no worries. near everyyear all the guppies will die out bar a few.
if you get some gambusia in your guppy ponds none will live the winter but the gambusia.
what will kill them is ferocious bubbling in cold water but they aure are strong. ive seen em rip at tadpoles in groups and they will chew the fins of goldfish if kept with them.
as for developing cold water strains of guppies. ive found it dont work well. i can keep one strain through a winter and breed the next year and you dont get much more surviving that winter but you do get a lot of undesirable colour and badly shaped tails so you need to introduce new stock to beat this. its not like a winter kill out results in much to select from in way of breeding colours and good tails see.