I don't see anyone really selling freshwater here. Whenever I feel I am bored with fresh I realize I am limiting my self too much. 400 gallons is a great tank. To be fair it would be an amazing reef tank as well.
A breeding pair of large cichlids like Argenteus, or Pearsei would be a lot of fun in that tank. You would never hurt for entertainment. Some large dithers like BA tetras, red hooks, or even a large school of medium size tetras like Colombian red and blues would be great. If you don't mind crossing borders in the tank large barbs work well mascara barbs, rohani barbs, or red panda barbs all would look good with that. If you want to keep a group of cichilds then panda uaru would be great in there as well.
If you want a more predatory tank you can go with large knives, Datnoids, or a bichir community. Don't rule out a large planted tank withe more peaceful inhabitants. Big mormyrids probably wouldn't disturb the plants. African arowana is another great fish. It might be a little tight in there but it will take a long time before it grows quite that large.
Tanganyikans are another option. A huge school of Cyps, a group of mustache cats, some shell dwellers and julidochromis would look great. Maybe try some ellipsifer eels. Or you can go for the more standard fish of Tanganyika like fronts, tropheus, and opthalmotilapia. I think your tank is a little small for them but boulengerochromis microlepis are cool too.
I will always love archers. A huge school of toxotes blythii in there would be awesome. The won't bother short plants and the bottom is free for whatever you want. I recommend a large school of burmese botias and a school of lake inle danios.
The recommendation of native fish isn't a bad one either. I think a native biotope following some of the Florida freshwater springs or spring discharges would be beautiful. Google pictures of Ginnie, Poe, Manatee, or Silver Glen Springs. You would make some fake cypress knees, use limerock and a sand bottom. Then plant some jungle vallisnaria, keep a large school of small shiners (blue nose are the best, can be legally acquired outside of Florida from Louisiana). Have a bull head catfish or two and a breeding pair of shell crackers, or any other panfish you like. Maybe even try to keep some hogchokers, or anguilla eels.
At the end of the day it is your tank. Whatever you do enjoy it. I can't deny it would be a nice reef tank as well.