Well, if you did a 100% change...and if your source water has zero nitrate...then a zero reading might make sense. Otherwise, I couldn't resist the temptation to test again, just to see if I got a reading that seems explainable.
If you did only a normal partial water change, and if you don't have have some ultramodern whizbang gizmo that is 100% effective at removing nitrates (pretty sure that doesn't exist!) then, yeah, do the test. If it reads zero again, something is screwy somewhere and may help point you in the direction of an explanation of this fish death.
Think about it. Whether your tank is cycled or not, how could there be fish living in it...eating, metabolizing, excreting waste...and yet all three parameters (ammonia, nitrite, nitrate) register as zero? Just doesn't add up.
