Let me start by making 2 statements:
- Most people do not need CO2 (lighting is much more important)
- Pressurized CO2 is so much easier (easier to get the rate right, consistant).
That being said, I do still have 1 tank running DIY. It is a planted feeder guppy 30. There are a lot of videos on YouTube that may give your better info than I can communicate here. You will have to decide how efficient you want your system to be. As mine is on a 30 that is fairly heavily planted and has HO t5 lighting, I needed a powered diffuser to get the CO
2 I needed.
First the assembly, a did a quick exmple for photos.

The first image is the container I used to make a drop checker. It is a Red Bull energy shot bottle.

Now for the tubing assembly you can use regular aquarium tubing or silicone based (CO
2 resistant) tubing. I used regular as the CO
2 will break down and harden the plastic, so it will all need to be replaced periodically. Drill your holes in the lids with a bit slightly smaller than the tubing and you can get a good pressure/friction fit. As stated before, the parts will have to be replaced periodically, so I dont worry about sealing with silicone or anything like that. It will hold more pressure than normally produced with no sealant if you get your fits right. Make your line running from your CO
2 generator (I assume you are using yeast fermentation as a DIY) to your drop checker long enough on the drop checker side to reach the bottom on the vessel. You can count the bubbles per minute to determine output. On the generator side, it should be as short as possible, just coming .25 to .5 inches into the bottle. Cut the tubing at an angle an pull through with pliars. The drop checker should be filled about 1/3 with clean water. Prevent any contamination. Most bacteria are hardier than yeast and will outcompete and kill it. The drop checker is important so you can judge how much CO
2 is being produced and change your culture.

The connector in my image should be replaced with a check valve. This will prevent tank water from being siphoned or forced by a pump back into the yeast vessel.

For my diffuser/reactor I wanted a powered setup that was small enough I could hide it in the back of the tank. I found a neat way of doing this with a bird feeder as in this image. Just drill a hole near the top and insert the output of a small fountain pump.

You will have to have some sponge in the open end to keep the CO
2 in the reactor until it can be absorbed or much of it will just be blown out.

Here is my assembly in tank. I had to move it around some to get a good shot, but it is usually hidden. Inline just runs into the fitting on the pump output, or you could connect it to the top of the reactor.
Now for your other question. On or off at night depends on some factors:
- How much CO2 is being produced?
- Alkalinity of the water (buffer).
- How much surface agitation you have.
My pump is on a timer and shuts off at lights out. CO
2 can drop pH significantly, so you will have to see for your self. If you are using a simple diffuser, them I'm guessing the system will not be efficient enough to really make a big difference as long as you have some surface movement. Having a method to check CO
2 levels is important.
I hope this helps and sorry if I photo bombed the thread. I could not think of a way to communicate it any other way. If you have more questions just ask, but I am soon to be out of the DIY CO
2 game. I have a small pressurized system in design now for this tank.
