How long has this tank been running, what size is it, and what stock do you have in it?
Agree to the above, you need a big water change to knock the ammo down, and then readd water with a good dose of Prime too. After it sits 10 minutes add your seeded bacteria, I only say 10 minutes because I like to give the Prime a little extra time first. If you want to use a liquid bacteria, Seachem Stability has worked for me, shake it until it looks milky. At this point follow the new tank directions on the bottle.