Help! fungus ID in brain and skin of cichlid

  • We are currently upgrading MFK. thanks! -neo
Dear TWG: According hippurate hydrolysis, the expert in LAB supplies recommend me a PCR LAMP. https://www.neb.com/en/products/m1804-warmstart-colorimetric-lamp-2x-master-mix-with-udg
But it is so expensive 646 USD per 100 reactions. Do you recommend other cheap option to perform hippurate hydrolysis? Best regards.
Unfortunately I'm not sure what is available to you so it's hard to give a solid recommendation. You can try asking what your lab members use if they have to use a cheap alternative.

I recall ninhydrin reagent being used as an alternative but I have never used it for hippurate hydrolysis of S. agalactiae. You'll have to search yourself what the exact steps are to use it.
 
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