We use Purigen in my daughters Axolotl tank and recharge it every few weeks. It slowly turns brown as it is saturated with nitrogen compounds from the tank water... so I assume it must be doing something. BUT we have never taken an analytical approach to try to determine how effective it actually is. I.e. take water measurements while the Purigen is out for a day recharging then see if the readings fall noticeably after putting it back into the filter.