I add have the prime before filling up and the rest while filling.Don't know if this is the best or even the right way off doing it but works for me
Add Prime before refilling. Or right after. Or during. It really doesn't matter, unless you have chloramines.
Matt
so my bacteria wont be wiped out?
As soon as I start filling my tank with my python I add my prime directly into the water flow, that way it gets mixed in evenly as quickly as possible.