I always add Prime, even though I have well water haha. The media is seeded, and once the sumps up the media in it will also have already been seeded. I'm honestly thinking it may be a PH issue now....because if I remember correctly Quikrete has limestone in their sand products...and I can't believe I didn't think about that beforehand...I normally fast cycle all my tanks...and with 310 gallons the bioload I'm putting in shouldn't stress the bacteria by any means but I will let it cycle out with some feeders in the tank and see how they do. Thanks everyoneA couple of things. Did you add any prime or safe to the water after you filled It? Was the water the same temp and Ph as the holding tank (acclamation)? The tank is not cycled if the filter wasn't seeded but I agree that's not the issues here because in 15 mins the cycle hasn't even started. I would cycle the tank for a couple of weeks using seeded media from an established filter and a couple of "clean" feeder goldfish or similar. Then acclimate any new additions properly adding only one fish at a time over the course of a couple of weeks so your biological filtration (beneficial Bacteria) can catch up to the bio-load of the fish & waste.