how often and how much water do you change in your 100gallon? If your tap water is a zero(prob 5ppm) and ya did a 75% W/C that will drop your nitrates down to around 20ppm. Then ya need to monitor daily and when it hits 40ppm do a 50% change to get back to 20ppm. once you get the rate of how quickly your nitrates rise, you can tailor your W/Cs and even work on getting the levels down to 10ppm.