I have 2 tanks with me that aren't so new. My first tank is a 40 to 50 Gallon tank that has 1 Red-Tip Arowana and a Janitor fish. My second tank is a small 15 to 25 gallon tank that has a Flowerhorn named Camillia. I just heard about Nitrogen Cycles in a Flowerhorn related thread that I've started before. Both of the tanks aren't new. The first tank is now with me for about 2 years now, and the other tank had just been used ( But it was given to me about 1 year ago ). I'm confused that I need to do a Nitrogen Cycle on both my 50 Gallon tank and my 25 Gallon tank. It isn't my first time using both of the tanks. It also isn't my first time keeping fish in them either. Do I have to do a Nitrogen Cycle on both my 50 Gallon tank and my 25 Gallon tank?