Dr. Joe,
What environment do you put those in to create anaerobic bacteria? You need a very low oxygen environment to produce facultative anaerobes. Maybe if you buried them under a sand substrate....
Might be an interesting experiment to place a sponge-foam mat on the bottom of a tank and cover it with a few inches of sand. The sponge may offer more low-oxygen surface area than sand alone; especially if the anaerobic bacteria starts foaming right at the transition line between sand and sponge.