Possibly, but I doubt it. I drove 9hrs with my fish (snooks included) in some rubbermaid tubs, then just netted them and added them to their new tank. No acclimation or anything. The water parameters were slightly different, and the water in the tank was a little warmer. Point is that unless there was a DRAMATIC change in parameters, or the fish were added to COLDER water, there shouldn't of been that much of a problem.