nitrate tests are done as directed in the API Kit, and i shake all bottles hard before i use them. As a side note i have tested my friends tanks with the same kit and returned nitrates, so i know the kit works and is not contaminated.
As for my media a use a special type by Cermedia which isnt commonly used and is dismissed a lot, this has been proven time to provide a sufficient, slow moving, oxygen free platform for Nitrate removing bacteria to grow, see this link http://www.cermedia.com/marinepure-technical.php
As per the instruction table in the above link for a 100gal tank you need 1 block, I have 12, according to my conversations with te CerMedia technical support, it takes 3-5 months to cultivate enough bacteria to efficient Nitrate removal, which i have to say i concured with, my particular tank took 12 weeks to see nitrates stop increasing between water changes, then after another 4 weeks my nitrates started to get less per week, and 4-5 weeks after that i started finding that there was no nitrates at all being detected.
As for my media a use a special type by Cermedia which isnt commonly used and is dismissed a lot, this has been proven time to provide a sufficient, slow moving, oxygen free platform for Nitrate removing bacteria to grow, see this link http://www.cermedia.com/marinepure-technical.php
As per the instruction table in the above link for a 100gal tank you need 1 block, I have 12, according to my conversations with te CerMedia technical support, it takes 3-5 months to cultivate enough bacteria to efficient Nitrate removal, which i have to say i concured with, my particular tank took 12 weeks to see nitrates stop increasing between water changes, then after another 4 weeks my nitrates started to get less per week, and 4-5 weeks after that i started finding that there was no nitrates at all being detected.

