I have a 240 gal which im using Just straight tap water from my sink. No Conditioner. So If they lived the first time im guessing i can do this again? How do u ppl Condition Big tangs? i have 3 smaller tank and i just fill a 5g bucket add a few drops and wait then add.. I love to do this for the 240 but after test one i dont see the point anymore. Plus i dont want to Fill 50 buckets and just slowly fill the thing back up. :X