Hello; I looked at the first post again and did not see the dimensions of the room, so will use a room of 12 feet by 20 feet. I suspect this is a fairly average sized room. I know that it is unlikely that someone would ever place 200 pounds on each square foot of a room, but the room would have to support 48,000 pounds if they did.
Another way to look at it. I have a boat that has held a 200+ pound friend without a problem, but it is rated for only a couple, maybe three, of those sized fellows in total.
It may also be that the tank will not be the only thing in the room. There may be furniture and other things, plus some number of people standing in front of the tank from time to time. I am not suggesting a load approaching 48,000 pounds, but a load of some size.
Should the OP decide to go ahead without additional support, I will be interested to know how it works out whichever way it goes. Please keep us posted.