I like deep sand beds and pothos vines, etc, personally. But to get anaerobic activity in lava rocks, make sure to get very light, fine-pored rocks, not the glassier heavy ones. Alternatively, a media does exist which does this very thing. I forget the name, but it's a large grade of media, like stones, meant for ponds. Somebody will chime in. But the answert is - Yes, anaerobic denitrification works well in freshwater. It's a matter of application.