I work for a General Contractor and we put a back lit glass floor in a recent job we completed. Profile of the glass was 2 layers of 1/2 inch, 1 layer of 3/8 inch, and a 1/4 layer with a anti-slip surface. All layers laminated with a PVB interlayer for strength. Basic module was 5 ft by 5 ft nominally. There was also colored inter layers for aesthetic purposes. Total glass thickness was 1 3/4" nominal when all said and done. The pieces were approximately $200/sf when shipped from LA to DC. Note that this was in a public assembly building and it is engineered for heavy traffic. Not sure if you could get away with a single thick piece or not. Either way, I would think it would be cost prohibitive. PM me if you want to know who we bought it from.
It comes down to how far apart the supports are. In several museums they have glass walkways over exhibits. As long as you are willing to put a wood/steel grate under the glass, you shouldn't need such thick glass. I have stood on the bottom of my 90 when it was upside down and it is fine. It is 1/2 inch glass, 4x2x2, or something pretty close to that. I realize this is an ancient thread, but it can be done. Maybe in the future someone will do it and there will be pics. I think a koi pond with something like this over part of it would be awesome.