A couple of things, paison. When you get the bottle of Stability, the spores will have formed a transluscent sludge on the bottom of the bottle. Find a clean container and pour off half the liquid...save this. Vigorously shake the Stability bottle for ten seconds or so to completely resuspend the spores. Replace the liquid and invert the bottle several times to disperse the spores. You are now ready to inoculate your tank. If you decide to use the cane sugar, I would suggest a ratio of 1 heaping teaspoon per 100 gallons. Don't get carried away or your water will turn cloudy. Finally, I left the fish in the tank overnight to build up some ammonia before adding the Stability in order to give the bacteria something to sink their teeth into. This worked well for me.