Absolutely NOT. Please stop giving advice on this.
If you aren't dosing your new water in seperate buckets outside of the tank (you aren't if you are doing water changes with a python), dose for the WHOLE TANK VOLUME.
If you don't know your water parameters, it's necessary to do this. Obviously you can tailor your dosing if you know what your city provides you with, but in that case, you wouldn't be making a thread like this.
Gee, what do I know but wouldn't it make a LOT more sense to quit asking strangers on an online chat forum, and actually find out what ones local water supply uses as a disinfectant, and at what level, and then treat accordingly?
Is this such a difficult concept for people to understand?
The OP could have anywhere from 0.3 ppm chlorine, to 3 ppm chloramine, coming out of his tap. Half the recommended dose on a Seachem label could be more than enough, or not nearly enough - and no one on this forum can give any kind of accurate response as to how much the OP should or shouldn't be using unless they know what his local water supply contains when it comes out of his taps.
The person that was just told to stop giving advice, actually gave the best advice in this discussion earlier on.
kingofthejungle
If you really wany precision you need to get your tap water levels checked then dose according to the results of your test.
That response, is the ONLY correct response.