looking for arowana breeding info
- Thread starter johnptc
- Start date
I have e-mailed a local lab here in Plyouth Meeting/PA, they sex birds via DNA in a feather for about $100, 99.99% accuracy. I want to know if they can sex fish as well, if so I will post.
Rod
Rod
Miguel;771164; said:If Delgado says there are no theories, then there are no theories...
i mean cmon guy how can one prove that you can visually sex an arowana every time and be correct every time?
DeLgAdO;771173; said:i mean cmon guy how can one prove that you can visually sex an arowana every time and be correct every time?
If you had read what i wrote, you would see that I wrote that there are theories, none absolute..and theories are just that, theories...there is no proven way to sex them before they breed.
I got a new theory...You can identify females because they have drop eye. Ya all the females have DE.
OK put the flame trowers away I'm just kidding.
It would be interesting to see if that lab could use Dna to identify the sex. I woulg assume they could get this from a scale.
OK put the flame trowers away I'm just kidding.
It would be interesting to see if that lab could use Dna to identify the sex. I woulg assume they could get this from a scale.
DeLgAdO;771173; said:i mean cmon guy how can one prove that you can visually sex an arowana every time and be correct every time?
U can't. It's possible to make educated guess but it's still a 50/50.
Hi All,
I have only recently discovered this site, heaps of good info guys and am still working through all the forums.
I have no experience with this specifically but have spent a few years in labs/field working in conservation genetics with relation to endangered fish along the following lines. But if you are interested in sexing an Arowana by using DNA you may wish to read this journal article:
Aquaculture Reasearch Volume 34 Issue 11 Page 951Issue 11 - 957 - September 2003. A strain-specific and a sex-associated STS marker for Asian arowana (Scleropages formosus, Osteoglossidae) G H Yue, D Ong, C C Wong, L C Lim, L Orban (2003).
This has about an 82% efficiency rate in sexing in Greens. I have not read this article in full but if you are serious about it an experienced lab may be able to use this info to design primers for PCR for silvers etc. they would hopefully be not to far apart. perhaps the researchers have done more work in this area since 2003 but not yet published and it might be worth talking to them. You never know you may be able to post them a frozen blood sample in anticoagulant.
Another interesting site with regard to Arowanas is http://www.arowanaclub.com/ a Singapour based group (mainly about Asian Aros but has a little bit about breeding and commercial pond setups).
Good luck.
I have only recently discovered this site, heaps of good info guys and am still working through all the forums.
I have no experience with this specifically but have spent a few years in labs/field working in conservation genetics with relation to endangered fish along the following lines. But if you are interested in sexing an Arowana by using DNA you may wish to read this journal article:
Aquaculture Reasearch Volume 34 Issue 11 Page 951Issue 11 - 957 - September 2003. A strain-specific and a sex-associated STS marker for Asian arowana (Scleropages formosus, Osteoglossidae) G H Yue, D Ong, C C Wong, L C Lim, L Orban (2003).
This has about an 82% efficiency rate in sexing in Greens. I have not read this article in full but if you are serious about it an experienced lab may be able to use this info to design primers for PCR for silvers etc. they would hopefully be not to far apart. perhaps the researchers have done more work in this area since 2003 but not yet published and it might be worth talking to them. You never know you may be able to post them a frozen blood sample in anticoagulant.
Another interesting site with regard to Arowanas is http://www.arowanaclub.com/ a Singapour based group (mainly about Asian Aros but has a little bit about breeding and commercial pond setups).
Good luck.
