I dont think females miss their male. They are rapers! First thing if you need to separate always the less stressed animal. Better way you make a seperation to split the tank this works only if you also have enough space to do that . Imagine getting first brutally raped, beaten up,waterboardered, and then get kicked out of your home. Besides that think of the bacterial fauna of the goldfish/guppys you have in your back up tank. its risky to confront a stressed ray with a new bacterial fauna. Try to get her in good condition feed her up keep her away from stress! A good active breedermale must be put in action wisely,otherwise it can do a lot of damage. Put him from time to time with 2 strong females in a sufficient big and cycled tank, let him do his tang put him out after 2-3 days.Bada bing bada bang call yourself breeder. Best you connect the tank of the females an the males so the problems of individual bacteria fauna doesnt occur they got the same bacterial fauna.