the beneficial bacteria live on surfaces inside the tank and filter. they are not 'free floating' in the water. doing a large water change will not affect the bacteria numbers.
0.5 to 1 ppm ammonia is deadly for fish. do a minimum 75% water change right now. use Prime. a 25% change will only remove 25% of the ammonia, so if your ammonia is at 1 ppm, then after a 25% water change you still have 0.75 ppm ammonia, which can be lethal to fish.
as for the test kits, did you get the dip strips or the liquid test kits that use drops of regents and vials?
as for davdev's comment about the 'bacteria in bottle' (Cycle) being a necessity, no it isn't. as long as you keep the ammonia and nitrites at zero, and do those water changes, your fish will not be in danger. correct on the not adding of any more fish now though.
0.5 to 1 ppm ammonia is deadly for fish. do a minimum 75% water change right now. use Prime. a 25% change will only remove 25% of the ammonia, so if your ammonia is at 1 ppm, then after a 25% water change you still have 0.75 ppm ammonia, which can be lethal to fish.
as for the test kits, did you get the dip strips or the liquid test kits that use drops of regents and vials?
as for davdev's comment about the 'bacteria in bottle' (Cycle) being a necessity, no it isn't. as long as you keep the ammonia and nitrites at zero, and do those water changes, your fish will not be in danger. correct on the not adding of any more fish now though.