I would leave the bio wheels because the Aerobic bacteria thrives on the wheels. If you don't have the wheel then all your aerobic bacteria will live in the substrate and the filter, which would depleat the Oxygen source from the water and thus maybe (depending on stock) deprive fish of oxygen. Aerobic bacteria needs Oxygen to live and ANaerobic bacteria does not. Aerobic bacteria processes ammonia and nitrites and ANaerobic bacteria converts nitrates to nitrogen gas which then escapes the tank via the water surface. The bio wheel does have (IMO) good surface area for bacteria BUT the constant exposure to air and water allows the Aerobic bacteria to perform at its best because it has more oxygen to thrive. The bio wheel is the main reason why I like the emp. 400 over filters like the Aquaclear. The aquaclear is a great filter but the emp. 400 has the wet/dry aspect with the bio wheels. So the moral of the story, If you want better water quality leave the bio wheels. That is my two cents
