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The ciliate protozoan, Ichthyophthirius multifiliis, and the fungus, Saprolegnia parasitica, cause the diseases ichthyophthiriosis and saprolegniosis respectively. Both diseases are difficult to control and can cause high mortalities of freshwater fish, including the Australian silver perch, Bidyanus bidyanus (Mitchell). The efficacy of salt (NaCl) in controlling and preventing these diseases in silver perch was evaluated in aquaria and tanks. Low pH levels were also evaluated as a control for ichthyophthiriosis. Concentrations of 2 or 3gL super(-1) salt controlled infestations of I. multifiliis, and fish were free of both theronts and trophonts by day 8 at temperatures of 17.3-21.3 degree C and by day 6 at 19.2-23.5 degree C. Fish treated with 1gL super(-1) salt remained infested and all fish in a control treatment (0gL super(-1) salt) died. Although the mean survival rates of infested fish at pH levels of 5 or 6 were only 13.9% and 7.6%, respectively, there were no theronts or trophonts on surviving fish after 12 days. Silver perch harvested from a pond and treated with 2 or 3gL super(-1) salt did not become infected with S. parasitica and survival was 100%, whereas 16.6% of untreated (0gL super(-1) salt) fish became infected and survival was only 66.7%. A concentration of 2gL super(-1) NaCl is recommended for the control of ichthyophthiriosis and the prevention of saprolegniosis in silver perch held in tanks, aquaria and re-circulating aquaculture systems.
A 1-year study was conducted from February 1999 to January 2000 to determine the effects of low levels of salinity on selected hematological parameters (total protein, hematocrit, leukocrit, and osmolality) and the health of channel catfish Ictalurus punctatus reared in ponds managed under the multiple cropping production strategy. We stocked 2,000 catfish into 15 ponds (0.08 ha each) that we subdivided into five replicate ponds per each of three NaCl (salinity) treatments: freshwater conditions (nominal salinity = 0 g/L of water) or adjusted salinities of 1.5 or 3 g/L. Total protein, hematocrit, and plasma osmolality for fish reared at the NaCl-induced salinities were significantly higher than those for fish cultured in freshwater. Although the leukocrit of fish reared at a salinity of 3 g/L was significantly higher than that of fish reared in freshwater or at 1.5 g/L, all were within the normal ranges reported for channel catfish. Although two bacterial diseases known to routinely affect channel catfish (enteric septicemia of catfish and motile aeromonad septicemia) were observed at all salinity levels tested, columnaris disease was not observed during the study. Parasitic infections during certain months were less severe for fish reared in saline ponds than in freshwater. The results indicate that the hematological variables measured in this study were higher at increased salinities but within the ranges reported as normal for channel catfish.
Three cases of severely compromised fish health and death in newly commissioned aquaculture facilities with water-recirculating systems are described. The cause of the damage and death was increased concentrations of water-borne nitrites and the subsequent methaemoglobinemia. The aim of the study was to better understand the aetiology of these cases of poisoning to help prevent them, and to examine effects of some water quality parameters on nitrite toxicity. The increased NO2- concentrations in water were caused by impaired functionality of biological filters in the second stage of nitrification, i.e. the conversion of NO2- to NO3-. Chloride concentrations in water
were considered the main factor influencing NO2- toxicity in all of the cases described. In the case of death of catfish and tench, the Cl- to N-NO2- weight ratios were in the range of 13 - 28 and 11 - 19, respectively. In the case of tilapia health impairment without symptoms of toxicity, the ratios were between 50 and 150. In the water tank inflow, the Cl- to N-NO2- weight ratios were between 2000 and 10000. Blood methaemoglobin levels of catfish and tench (severe symptoms of poisoning) and of tilapia (no signs of impairment, only brownish discolouration of gills) were over 80% and 21%, respectively). In order to minimize risks in culture of fish in water-recirculating systems, it is necessary to choose a proper stock of fish and a proper feeding ratio, not to treat the fish with antibiotics in the form of baths, to check meticulously the quality of water. In case of increasing concentration of nitrites, to administer sodium chloride to get the chloride concentration increased at least to 100 mg·l-1 Cl-. Better operation of a biological filter can be speeded up by inoculation with activated sludge
Channel catfish ponds are treated with salt (sodium chloride) to increase chloride concentration and prevent nitrite toxicity in fish. A survey indicated that most farmers try to maintain chloride concentration of 50 to 100 mg/L in ponds by annual salt applications. Averages and standard deviations for selected water quality variables in salt-treated ponds were as follows: chloride. 87.2 ± 37.5 mg/L; total dissolved solids (TDS), 336 ± 96 mg/L; specific conductance, 512 ± 164 μmhos/cm. Maximum values were 189 mg/L for chloride, 481 mg/L for TDS, and 825 μmhos/cm for specific conductance. Good correlations between specific conductance values and both chloride and TDS concentrations suggest that specific conductance can be a rapid method for estimating concentrations of these two variables in surface water. The maximum limit for chloride concentration in Alabama streams allowed by the Alabama Department of Environmental Management is 230 mg/L. The usual recommended upper limit of TDS for protection of aquatic life in freshwater streams is 1,000 mg/L. Based on the observed relationship between TDS concentration and specific conductance in Alabama catfish ponds, 1,000 mg/L TDS corresponds to 1,733 μmhos/cm specific conductance. It is unlikely that effluents from salttreated catfish ponds would violate the in-stream chloride standard of 230 mg/L or harm aquatic life in streanis. Nevertheless, chloride concentrations in ponds should be measured before salt application as a safe guard against excessive salt application and chloride concentrations above the in-stream chloride standard.
Static tests were performed to determine the acute toxicities of formalin, chloramine-T, and sodium chloride on juvenile Atlantic sturgeon Acipenser oxyrinchus (average weight, 2.1 g) in oxygen-saturated dilution water (total hardness, 86.5 mg/L) at 17°C. The concentrations that were lethal to 50% of the test fish over a 96-h period (the 96-h LC50 values) were 31.00 μL/L for formalin, 7.73 mg/L for chloramine-T, and 9.735 g/L for sodium chloride. These findings indicate that the therapeutic concentrations and exposure times that are commonly employed in aquaculture would not be acutely toxic to Atlantic sturgeon
Small (4.4 ± 1.50 g; mean ± SD) Nile tilapias Oreochromis niloticus were more tolerant of nitrite than large (90.7 ± 16.43 g) fish. The 96-h median lethal concentration of nitrite-N to small fish was 81 mg/L (95% confidence interval = 35−127 mg/L) compared with 8 mg/L (4−11 mg/L) for large fish. Addition of chloride to test water (as either calcium chloride or sodium chloride) protected both small and large fish from nitrite. Sodium chloride and calcium chloride appeared to be similarly effective in inhibiting nitrite toxicity.
Four species of Australian warmwater fishes were treated for ichthyophthiriasis with 5 g common salt/L. Juvenile silver perch (Bidyanus bidyanus), golden perch (Macquaria ambigua), Murray cod (Maccullochella peeli), and catfish (Tandanus tandanus) recovered from infestations of Ichthyophthirius multifiliis within 2 weeks. All fish in the control treatments died. The treatment eliminated the theronts and trophonts from all host fish by day 7 within the temperature range 1926°C and by day 14 within the temperature range 1118°C