Yesterday I did another 20% water change and added a bubbler.1. Retest your parameters.
2. Do not rinse media in fresh water always use old tank water.
3. 80f is to hot for guppies they can handle it some times but they are more temperate fish not tropical.
4. Agree possible parasite or disease added with new fish.
5. Also agree that with that long of an established tank a wc wont wipe out enough bacteria to cause a recycle. Its in the substrate, on the decor, in the filter on the glass. You would have to remove everything let it all dry out for days or bleach it to kill all the bacteria.
today I just re-tested and moved my seachem ammonia alert into the tank not sure how long it will take for the ammonia alert detector to kick in but I’ll give it a few hours to adjust.
todays year results.
Ammonia - between 0 and 0.25 ppm
NI - 0
NA - 0
Today I will be turning down the heater to get a temp of about 78.