RD, your killing me man!!! Did you even read the abstract?
"Gizzard shad thiaminase activity and its effect on the thiamine status of captive American alligators"
What a load of misleading conjecture. BTW, the gators that were tested for thiamin deficiency were held captive for up to 10 months and fed dead shad three times a day. Healthy thiamin levels from gators in the lake were used as the benchmark to compare against. Its hard to say 7 years later what caused the die-off in the lake. But, we know from mink that if you feed an exclusive diet of thiaminase containing fish that is dead and or frozen thiamin deficiency will occur after a very prolonged time (many months to years).
I'll get back to you on the gators later tonight. There may be some other points worth responding to as well.
Still no willingness to kick it around about setting up a growth trial. My dime. Nothing to loose, right? And, maybe we could learn a little too. I want to be sure that the canned diet is handled properly so that the trial fish get the best possible chance. How do you get small fish trained onto a pellet? What would you recommend in terms of feeding frequency. How long would you want to see uneaten feed left in the tank before removal. etc... What species would you recommend for a trial. I don't want something that is too finicky or difficult to raise. It would need to be a staple of any aquarium store. Help from any source is welcome. We'll have months to chat up the topic while I set up the trial and conduct it. Since in the "captive gator" trial 6 months was enough to show thiamin deficiency and lethargy, 6 to 10 months would be long enough for a trial. Longer if you think that would be needed. I really appreciate your input. Maybe we can turn your enthusiasm towards getting some data points.
Thanks,
Rich