DNA tests were commissioned by Jeff Rapps in August of 2005 in an attempt to verify the authenticity of Hector Luzardos claim. The results of the test furnished by Dr. Prosanta Chakrabarty proportedly showed that Electric Blue Jack Dempsey are indeed a color morph of octofasciata.
Many people in the cichlid community were doubtful over these results, and proclaimed the tests were run incorrectly and only showed mitachondrial DNA. Further investigation into this found that Dr. Prosanta Chakrabarty did indeed run nuclear DNA tests, and simply neglected to share them with people.
In an attempt to verify the results of these tests, members of the bluejax forum (
http://bluejax.14.forumer.com/index.php) came together to help me fit the bill for some DNA analysis. Our DNA tests proved the same, that no other DNA was found in the gene region we targeted. Here is a copy of that test:
EBF8 = electric blue female
EBM8 = electric blue male
JDF8 = jack dempsey female
JDM8 = jack dempsey male
SEQ EBF8-20-07-10-41 AM: 504 bp;
Composition 109 A; 109 C; 133 G; 153 T; 0 OTHER
Percentage: 21.6% A; 21.6% C; 26.4% G; 30.4% T; 0.0%OTHER
Molecular Weight (kDa): ssDNA: 155.89 dsDNA: 310.71
ORIGIN
1 TTTTACACTG GACTGGCTTT TCTGTTTTAA AATGTACTTT AATTGTGTTT AATGGCAAAA
61 TTTACACACA GTAAGGCATG TGAAGACTGT GATTCGTTAG ATTATGTGAG TTGCAGCACC
121 GGATGGCTCA TTTTTACGGC TAAGTAAGGC GTAGTAGAGC AGCTCCGGTC AATGAGTTTG
181 GGTTAGCGGG TAGCTAGCAG CAAGCAGCAG GCCGTGCATG TGTTCAGCTA GCCGCCACAG
241 AAGCGAGAAA AGGAGACCTT AAGTTCACTT TCAGGGCCGG AGGTTCAGAT TTTTTCATTG
301 TTGCTTATAT AGTTGTTTTG TAAGTTCCGC TATATGTCCA TGGTGCTGTT ATATCCATCT
361 GCTCAGCGCC TGTGAGCTAG CGCGGTGAGC TCTGGTGCTG CCCCTTTAGG TTAGCGCCGC
421 TCGCCCCGCA GCTCCTGGCT TTCAGTGAAG GATGGACGTT AAAGATGCTC CGCTGCCCTT
481 CAGCGGTTCT AACCCACATG TCTT
SEQ EBM8-20-07-9-44 AM: 503 bp;
Composition 109 A; 109 C; 133 G; 152 T; 0 OTHER
Percentage: 21.7% A; 21.7% C; 26.4% G; 30.2% T; 0.0%OTHER
Molecular Weight (kDa): ssDNA: 155.58 dsDNA: 310.09
ORIGIN
1 TTTTACACTG GACTGGCTTT TCTGTTTTAA AATGTACTTT AATTGTGTTT AATGGCAAAA
61 TTTACACACA GTAAGGCATG TGAAGACTGT GATTCGTTAG ATTATGTGAG TTGCAGCACC
121 GGATGGCTCA TTTTTACGGC TAAGTAAGGC GTAGTAGAGC AGCTCCGGTC AATGAGTTTG
181 GGTTAGCGGG TAGCTAGCAG CAAGCAGCAG GCCGTGCATG TGTTCAGCTA GCCGCCACAG
241 AAGCGAGAAA AGGAGACCTT AAGTTCACTT TCAGGGCCGG AGGTTCAGAT TTTTTCATTG
301 TTGCTTATAT AGTTGTTTTG TAAGTTCCGC TATATGTCCA TGGTGCTGTT ATATCCATCT
361 GCTCAGCGCC TGTGAGCTAG CGCGGTGAGC TCTGGTGCTG CCCCTTTAGG TTAGCGCCGC
421 TCGCCCCGCA GCTCCTGGCT TTCAGTGAAG GATGGACGTT AAAGATGCTC CGCTGCCCTT
481 CAGCGGTTCT AACCCACATG TCT
SEQ JDF8-20-07-8-47 AM: 500 bp;
Composition 109 A; 108 C; 133 G; 150 T; 0 OTHER
Percentage: 21.8% A; 21.6% C; 26.6% G; 30.0% T; 0.0%OTHER
Molecular Weight (kDa): ssDNA: 154.69 dsDNA: 308.24
ORIGIN
1 TTTTACACTG GACTGGCTTT TCTGTTTTAA AATGTACTTT AATTGTGTTT AATGGCAAAA
61 TTTACACACA GTAAGGCATG TGAAGACTGT GATTCGTTAG ATTATGTGAG TTGCAGCACC
121 GGATGGCTCA TTTTTACGGC TAAGTAAGGC GTAGTAGAGC AGCTCCGGTC AATGAGTTTG
181 GGTTAGCGGG TAGCTAGCAG CAAGCAGCAG GCCGTGCATG TGTTCAGCTA GCCGCCACAG
241 AAGCGAGAAA AGGAGACCTT AAGTTCACTT TCAGGGCCGG AGGTTCAGAT TTTTTCATTG
301 TTGCTTATAT AGTTGTTTTG TAAGTTCCGC TATATGTCCA TGGTGCTGTT ATATCCATCT
361 GCTCAGCGCC TGTGAGCTAG CGCGGTGAGC TCTGGTGCTG CCCCTTTAGG TTAGCGCCGC
421 TCGCCCCGCA GCTCCTGGCT TTCAGTGAAG GATGGACGTT AAAGATGCTC CGCTGCCCTT
481 CAGCGGTTCT AACCCACATG
SEQ JDM8-20-07-7-41 AM: 489 bp;
Composition 105 A; 104 C; 131 G; 148 T; 1 OTHER
Percentage: 21.5% A; 21.3% C; 26.8% G; 30.3% T; 0.2%OTHER
Molecular Weight (kDa): ssDNA: 151.32 dsDNA: 301.46
ORIGIN
1 TTTTACACTG GACTGGCTTT TCTGTTTTAA AATGTACTTT AATTGTGTTT AATGGCAAAA
61 TTTACACACA GTAAGGCATG TGAAGACTGT GATTCGTTAG ATTATGTGAS TTGCAGCACC
121 GGATGGCTCA TTTTTACGGC TAAGTAAGGC GTAGTAGAGC AGCTCCGGTC AATGAGTTTG
181 GGTTAGCGGG TAGCTAGCAG CAAGCAGCAG GCCGTGCATG TGTTCAGCTA GCCGCCACAG
241 AAGCGAGAAA AGGAGACCTT AAGTTCACTT TCAGGGCCGG AGGTTCAGAT TTTTTCATTG
301 TTGCTTATAT AGTTGTTTTG TAAGTTCCGC TATATGTCCA TGGTGCTGTT ATATCCATCT
361 GCTCAGCGCC TGTGAGCTAG CGCGGTGAGC TCTGGTGCTG CCCCTTTAGG TTAGCGCCGC
421 TCGCCCCGCA GCTCCTGGCT TTCAGTGAAG GATGGACGTT AAAGATGCTC CGCTGCCCTT
481 CAGCGGTTC
JDM-8-20-07 TTTTACACTGGACTGGCTTTTCTGTTTTAAAATGTACTTTAATTGTGTTTAATGGCAAAA
JDF8-20-07 TTTTACACTGGACTGGCTTTTCTGTTTTAAAATGTACTTTAATTGTGTTTAATGGCAAAA
EBM8-20-07 TTTTACACTGGACTGGCTTTTCTGTTTTAAAATGTACTTTAATTGTGTTTAATGGCAAAA
EBF8-20-07 TTTTACACTGGACTGGCTTTTCTGTTTTAAAATGTACTTTAATTGTGTTTAATGGCAAAA
DQ119255 TTTTACACTGGACTGGCTTTTCTGTTTTAAAATGTACTTTAATTGTGTTTAATGGCAAAA
DQ836808 ----------------CTTTTCTGTTTTAAAATGTACTTTAATTGTGTTTAATGGCAAAA
********************************************
JDM-8-20-07 TTTACACACAGTAAGGCATGTGAAGACTGTGATTCGTTAGATTATGTGASTTGCAGCACC
JDF8-20-07 TTTACACACAGTAAGGCATGTGAAGACTGTGATTCGTTAGATTATGTGAGTTGCAGCACC
EBM8-20-07 TTTACACACAGTAAGGCATGTGAAGACTGTGATTCGTTAGATTATGTGAGTTGCAGCACC
EBF8-20-07 TTTACACACAGTAAGGCATGTGAAGACTGTGATTCGTTAGATTATGTGAGTTGCAGCACC
DQ119255 TTTACACACAGTAAGGCATGTGAAGACTGTGATTCGTTAGATTATGTGAGTTGCAGCACC
DQ836808 TTTACACACAGTAAGGCATGTGAAGACTGTGATTCGTTAGATTATGTGACTTGCAGCACC
************************************************* **********
JDM-8-20-07 GGATGGCTCATTTTTACGGCTAAGTAAGGCGTAGTAGAGCAGCTCCGGTCAATGAGTTTG
JDF8-20-07 GGATGGCTCATTTTTACGGCTAAGTAAGGCGTAGTAGAGCAGCTCCGGTCAATGAGTTTG
EBM8-20-07 GGATGGCTCATTTTTACGGCTAAGTAAGGCGTAGTAGAGCAGCTCCGGTCAATGAGTTTG
EBF8-20-07 GGATGGCTCATTTTTACGGCTAAGTAAGGCGTAGTAGAGCAGCTCCGGTCAATGAGTTTG
DQ119255 GGATGGCTCATTTTTACGGCTAAGTAAGGCGTAGTAGAGCAGCTCCGGTCAATGAGTTTG
DQ836808 GGATGGCTCATTTTTACGGCTAAGTAAGGCGTAGTAGAGCAGCTCCGGTCAATGAGTTTG
************************************************************
JDM-8-20-07 GGTTAGCGGGTAGCTAGCAGCAAGCAGCAGGCCGTGCATGTGTTCAGCTAGCCGCCACAG
JDF8-20-07 GGTTAGCGGGTAGCTAGCAGCAAGCAGCAGGCCGTGCATGTGTTCAGCTAGCCGCCACAG
EBM8-20-07 GGTTAGCGGGTAGCTAGCAGCAAGCAGCAGGCCGTGCATGTGTTCAGCTAGCCGCCACAG
EBF8-20-07 GGTTAGCGGGTAGCTAGCAGCAAGCAGCAGGCCGTGCATGTGTTCAGCTAGCCGCCACAG
DQ119255 GGTTAGCGGGTAGCTAGCAGCAAGCAGCAGGCCGTGCATGTGTTCAGCTAGCCGCCACAG
DQ836808 GGTTAGCGGGTAGCTAGCAGCAAGCAGCAGGCCGTGCATGTGTTCAGCTAGCCGCCACAG
************************************************************
JDM-8-20-07 AAGCGAGAAAAGGAGACCTTAAGTTCACTTTCAGGGCCGGAGGTTCAGATTTTTTCATTG
JDF8-20-07 AAGCGAGAAAAGGAGACCTTAAGTTCACTTTCAGGGCCGGAGGTTCAGATTTTTTCATTG
EBM8-20-07 AAGCGAGAAAAGGAGACCTTAAGTTCACTTTCAGGGCCGGAGGTTCAGATTTTTTCATTG
EBF8-20-07 AAGCGAGAAAAGGAGACCTTAAGTTCACTTTCAGGGCCGGAGGTTCAGATTTTTTCATTG
DQ119255 AAGCGAGAAAAGGAGACCTTAAGTTCACTTTCAGGGCCGGAGGTTCAGATTTTTTCATTG
DQ836808 AAGCGAGAAAAGGAGACCTTAAGTTCACTTTCAGGGCCGGAGGTTCAGATTTTTTCATTG
************************************************************
JDM-8-20-07 TTGCTTATATAGTTGTTTTGTAAGTTCCGCTATATGTCCATGGTGCTGTTATATCCATCT
JDF8-20-07 TTGCTTATATAGTTGTTTTGTAAGTTCCGCTATATGTCCATGGTGCTGTTATATCCATCT
EBM8-20-07 TTGCTTATATAGTTGTTTTGTAAGTTCCGCTATATGTCCATGGTGCTGTTATATCCATCT
EBF8-20-07 TTGCTTATATAGTTGTTTTGTAAGTTCCGCTATATGTCCATGGTGCTGTTATATCCATCT
DQ119255 TTGCTTATATAGTTGTTTTGTAAGTTCCGCTATATGTCCATGGTGCTGTTATATCCATCT
DQ836808 TTGCTTATATAGTTGTTTTGTAAGTTCCGCTATATGTCCATGGTGCTGTTATATCCATCT
************************************************************
JDM-8-20-07 GCTCAGCGCCTGTGAGCTAGCGCGGTGAGCTCTGGTGCTGCCCCTTTAGGTTAGCGCCGC
JDF8-20-07 GCTCAGCGCCTGTGAGCTAGCGCGGTGAGCTCTGGTGCTGCCCCTTTAGGTTAGCGCCGC
EBM8-20-07 GCTCAGCGCCTGTGAGCTAGCGCGGTGAGCTCTGGTGCTGCCCCTTTAGGTTAGCGCCGC
EBF8-20-07 GCTCAGCGCCTGTGAGCTAGCGCGGTGAGCTCTGGTGCTGCCCCTTTAGGTTAGCGCCGC
DQ119255 GCTCAGCGCCTGTGAGCTAGCGCGGTGAGCTCTGGTGCTGCCCCTTTAGGTTAGCGCCGC
DQ836808 GCTCAGCGCCTGTGAGCTAGCGCGGTGAGCTCTGGTGCTGCCCCTTTAGGTTAGCGCCGC
************************************************************
JDM-8-20-07 TCGCCCCGCAGCTCCTGGCTTTCAGTGAAGGATGGACGTTAAAGATGCTCCGCTGCCCTT
JDF8-20-07 TCGCCCCGCAGCTCCTGGCTTTCAGTGAAGGATGGACGTTAAAGATGCTCCGCTGCCCTT
EBM8-20-07 TCGCCCCGCAGCTCCTGGCTTTCAGTGAAGGATGGACGTTAAAGATGCTCCGCTGCCCTT
EBF8-20-07 TCGCCCCGCAGCTCCTGGCTTTCAGTGAAGGATGGACGTTAAAGATGCTCCGCTGCCCTT
DQ119255 TCGCCCCGCAGCTCCTGGCTTTCAGTGAAGGATGGACGTTAAAGATGCTCCGCTGCCCTT
DQ836808 TCGCCCCGCAGCTCCTGGCTTTCAGTGAAGGATGGACGTTAAAGATGGTCCGCTGCCCTT
*********************************************** ************
JDM-8-20-07 CAGCGGTTC---------------------------------------------------
JDF8-20-07 CAGCGGTTCTAACCCACATG----------------------------------------
EBM8-20-07 CAGCGGTTCTAACCCACATGTCT-------------------------------------
EBF8-20-07 CAGCGGTTCTAACCCACATGTCTT------------------------------------
DQ119255 CAGCGGTTCTAACCCACATGTCTTTATTTCGCCGTGCAGAGACAAAGGCCATGTTCAGTA
DQ836808 CGGCGGTTCTAACCCACATGTCTTTA----------------------------------
* *******
JDM-8-20-07 ---
JDF8-20-07 ---
EBM8-20-07 ---
EBF8-20-07 ---
DQ119255 CCA
DQ836808 ---